Page 93 - FINAL COMPENDIUM 2020-2021 27.2.2022

AN IN VITRO EVALUATION OF THE EFFECTS OF DIFFERENT PRE-TREATMENT PROTOCOLS
ON FISSURE SEALANT MICROLEAKAGE

Suharni P, Sabri M, Maryani MR
Objectives: The aim of this study was to evaluate and compare in vitro, the effects of
applying different enamel pre-treatments with conventional acid-etch, etch-and-rinse
adhesives, and self-etch adhesives on microleakage around fissure sealant margins in
primary and permanent molar teeth. Material and methods: Thirty extracted human third
molars and primary second molars were randomly assigned into three groups of ten teeth
each. Clinpro TM (3M, ESPE) sealant was applied after undergoing either one of these pre-
TM
treatment protocols; 1) 37% phosphoric acid etching (Scotchbond ); 2) 37% phosphoric
acid etching (Scotchbond ) + Prime & Bond (Denstply) and 3) Single Bond Universal
NT
TM
(3M, ESPE). Specimens were stored in distilled water for 30 days, thermocycled and then,
immersed for 24 hours in 0.5% basic fuchsin solution. Buccolingual slices of samples were
examined under stereomicroscope. The Kruskal-Wallis test was used for comparison
between three pre-treatment protocols. Results: There was a significant difference (p <
0.05) among three groups in both permanent and primary groups. In permanent and
primary teeth, acid etching technique showed the highest group of no leakage (96.7%)
(93.3%), followed by self-etch adhesive at (76.7%) (73.3%) and etch-and-rinse adhesive at
(46.7%) (63.3%) respectively. Significance: The use of phosphoric acid etching without
adhesive reduced fissure sealant microleakage.

Keywords: Fissure sealant, Microleakage, Acid etch, Adhesive, Self-etch adhesive, Etch-and-rinse
adhesive

Poster presentation at the National Conference Malaysian Dental Association Scientific Convention and Trade Exhibition,
SCATE 2020 on 14 – 16 February 2020

Dr Suharni Puteh
Paediatric Dentistry Department
Hospital Sultanah Nur Zahirah
Kuala Terengganu, Terengganu

Prof. Dr Sabri Musa
Dr Maryani Mohamed Rohani
Paediatric Dentistry and Orthodontics Department
Faculty of Dentistry
University of Malaya
Kuala Lumpur, Malaysia

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