Page 96 - FINAL COMPENDIUM 2020-2021 27.2.2022

ANTI-BIOFILM EFFECTS OF EFFECTIVE ECO-PRODUCE ON ENTEROCOCCUS FAECALIS

Ng MY, Zaleha S, Mariati AR, S. Sockalingam NMP, Ahmad Shuhud IZ, Alida M

Introduction: Enterococcus faecalis is a persistent bacterium in recurrent endodontic
infection. Sodium hypochlorite (NaOCl), the gold standard for endodontic irrigation has
several disadvantages. Effective eco-produce (EEP) which is an organic product from
kitchen waste, molasses, and water, mixed in appropriate ratio, is a well-known natural
cleansing agent. Objective: To determine the anti-biofilm effects of EEP under different
composition of source material and fermentation periods. Materials and methods: EEP
was prepared from different kitchen wastes, namely pineapple (P), orange (O), and a
mixture of pineapple and orange (M), and was fermented for three and six months,
respectively. Static batch growth microtiter plates anti-biofilm assay was carried out for
all EEP groups. Percentage of biofilm removal on microtiter plate was measured using
crystal violet staining, and compared with the positive control (NaOCl) using three-way
mixed ANOVA. Results: There was no statistically significant difference in anti-biofilm
effects between NaOCl and O-EEP at three- and six-months’ fermentation period (1.56%
concentration, p = 0.18 and 0.78%-3.13% concentrations, p = 1.00, respectively). For six
months old P- and M-EEP, no significant difference in anti-biofilm effects was found
(3.13%-12.5% concentrations, p = 0.09-1.00, and 6.25% concentration, p = 0.91,
respectively) in regards to that of NaOCl. Conclusion: In conclusion, six months old O-EEP
has the best anti-biofilm effects at 3.13% concentration towards E. faecalis. These results
suggest that EEP has the potential to be an endodontic irrigant, but further studies are
required.

Virtual oral presentation at the MAPD 2021 Virtual Scientific Conference on 8 March 2021.

Dr Meei Yi Ng
Paediatric Dentistry Department
Hospital Tengku Ampuan Afzan
Kuantan, Pahang
Malaysia

Dr Zaleha Shafiei
Dr Mariati Abdul Rahman
Centre for Craniofacial Diagnostics and Biosciences
Faculty of Dentistry
Universiti Kebangsaan Malaysia
Kuala Lumpur, Malaysia

AP Dr S. Nagarajan M.P. Sockalingam
Dr Ahmad Shuhud Irfani Zakaria
Dr Alida Mahyuddin
Centre for Family Oral Health
Faculty of Dentistry
Universiti Kebangsaan Malaysia
Kuala Lumpur, Malaysia

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